Western blot was performed using Anti-MSH6 Monoclonal Antibody (5B11) (Product # MA5-15917) and a 160kDa band corresponding to MSH6 was observed in all the tested cell models, except Jurkat and LNCaP which are reported to be negative. This target undergoes heavy phosphorylation and multiple bands between 120-160kDa can be expected. Modified whole cell lysate (1% SDS) (30ug lysate) of K-562 (Lane 1), Jurkat (Lane 2), HeLa (Lane 3), LNCaP (Lane 4), PC-3 (Lane 5), HCT 116 (Lane 6) and A549 (Lane 7) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).